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Confocal Microscopy Core


The Confocal Core Facility is located on the 2 nd floor of the new Arthur C. Guyton Research Building in Room G272. The Confocal Core houses a Leica TCS-SP2 laser scanning confocal upright microscope. The confocal system has 3 separate lasers for use with 3 standard visible length fluorescence dyes as well as transmitted light.

 

Contact Information:

 

Heather Drummond

Phone: (601) 984-1812

Email: hdrummond@physiology.umsmed.edu

 

 

 

Services/Capabilities/Fees

 

The confocal microscope equipped with three individual lasers (488/546/633 nm) capable of imaging standard fluorescence dyes within the visible spectra including FITC/TRITC, CY2/CY3/CY5, green fluorescence protein variants (GFP/YFP/RFP/DsRed) and many different indicator dyes (i.e. Ca 2+, pH, membrane potential, oxidative stress, etc).

 

Capabilities

  • Standard confocal microscopy with simultaneous or sequential image collection
  • Line scan, Z-scan modes
  • 10X, 20X, 40X, 40X oil, 60X oil and 100X oil objectives
  • Transmitted light detection
  • Tunable filter
  • Time lapse
  • FRET/FRAP (Fluorescence resonance excitation transfer/ Fluorescence recovery after photobleaching)
  • 3D Reconstruction

 

 

Fees

              Training: $100/hr

              User based imaging: $50/hr

 

The Confocal Microscopy Core is primarily an equipment core. HOWEVER, ALL USERS MUST BE TRAINED AND PRE-APPROVED PRIOR TO USE BY THE CONFOCAL CORE DIRECTOR. Users new to confocal microscopy typically require 2 - 4 hours of training depending on level of microscopy experience. Experienced users typically require 1 - 2 hours of training.