| CURRENT HPLC PROTOCOLS | |||||||
| Analyte | Volume required for duplicate determinations | Injected Volume | Minimum Detectible Level | Pre-processing required | Pricing | ||
| in sample | on column | method | dilution | ||||
| Creatinine MW = 113 | 5 μl
plasma or 0.5 μl urine (diluted up to 5 μl) | 25 μl | in
plasma: 10 μmol/L (0.1 mg/dl) | 5
pmoles (500 pg) | Acetonitrile
deproteination, lyophilization reconstitution and filtration (P.S.T. Yuen et al., Am. J. Physiol. Renal Physiol, 286: F1116-F1119, 2004. | 18-fold dilute | TBA |
| Norepinephrine MW = 169 | 1.2 ml plasma | 70 μl | 20
pg/ml (0.1 nmol/L) | 8
pg (50 fmoles) | Anticoagulation
with EDTA (Extraction done in HPLC facilities) | 6-fold concentrated | TBA |
| Leukotriene B4, 20-hydroxy LTB4 | 200 μl | 10 μl | 50 nmol/L | 0.5 pMoles | Extraction
on a C18 Sep-Pac and filtration (J. Bylund et al., Arch Bioch Biophys, 412: 34-41, 2003) | 200/150 - fold concentrated | TBA |
| Glutathione & Cyst(e)ine MW (reduced form) = 307 & 121 | 10 mg tissue | 20 μl | -- | 20
pMoles oxidized form; 100 pMoles reduced form | Derivitization
with dansyl chloride and filtration (D. Jones, Meth. Enzym. 348:93-112, 2002) | -- | TBA |